Journal: International Journal of Molecular Sciences

Article Title: The CXCR4–CXCL12 -Axis Is of Prognostic Relevance in DLBCL and Its Antagonists Exert Pro-Apoptotic Effects In Vitro

doi: 10.3390/ijms20194740

Figure Lengend Snippet: Growth inhibition and apoptosis of B cell lymphoma cell lines upon treatment with CXCR4 antagonists. ( a ) Structure of the CXCR4 antagonists AMD3100, AMD070, and WK1. ( b ) Cell growth of SuDHL4 (as GCB-DLBCL model), RI-1 and U2932 (as NGCB-DLBCL model), and BL2 (as Burkitt model) cell lines in the presence of increasing concentrations (range: 1–90 µM) of the CXCR4 antagonists AMD3100, AMD070, its niacin derivative WK1 and niacin, respectively, as determined by the EZ4U proliferation assay and expressed by percentage of normal absorption. ( c ) Annexin V positivity of BL2 (as Burkitt model) and SuDHL4 (as GCB-DLBCL model) cells treated with AMD3100, AMD070 and its niacin derivative WK1 (concentration: 40 µM; for 48 h) as determined by flow cytometry and compared to the DMSO treated control cells. The treatments and Annexin V staining were performed in triplicate and the medians ± standard deviations are depicted. ( d ) Percentage of cleaved caspase 3 positive BL2 (as Burkitt model) and SuDHL4 (as GCB-DLBCL model) cells treated with 40 µM of AMD070 or 20 µM and 40 µM of its niacin derivative WK1 for 24 h as determined by flow cytometry and compared to the DMSO treated control cells. The treatments and cleaved caspase staining were performed in triplicate and the medians ± standard deviations are depicted.

Article Snippet: All cell lines were treated with the commercially available CXCR4 antagonists (MedChemExpress, Sollentuna, Sweden) AMD3100 and AMD070 [ ], and the novel niacin derivative of AMD070 called WK1, which was generated by us, in a range from 1 µM to 90 µM.

Techniques: Inhibition, Proliferation Assay, Concentration Assay, Flow Cytometry, Control, Staining

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Concentration of AMD070 in the lungs of CD-1 mice at various times after PO administration at 400 μg/animal. Data represent the means (± SEM) of 3 mice. Horizontal dash line represents EC 90 of 44ng/mL. (SEM, Standard error of mean).

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Cell counts in the blood of CD-1 mice at various times after the PO administration of AMD070 at either 200 (A) or 400 (B) μg/mouse. Data shown are WBCs (●; x 10 3 /μL), RBCs (■; x 10 6 /μL) and platelets (▲ x 10 6 /μL) and are the means (± SEM) of 3 animals.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques:

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Differential cell counts in the blood of CD-1 mice at various times following PO administration of AMD070 at either 200 (A) or 400 (B) μg/mouse. Data are shown for lymphocytes (■), neutrophils (●), monocytes (▲) and eosinophils (▼) and are the means (± SEM) of 3 animals.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques:

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Graph in (A) represents the means (± SEM) of percent surface area with high inflammatory cell infiltrate, as measured by H&E staining intensity. Representative H & E stained lungs of PBS plus acetate buffer (B), BLM plus acetate buffer (C) and BLM plus AMD070 (D) treated mice. Black arrows indicate areas with increased inflammatory cell infiltrate. AB indicates acetate buffer and this notation is used for the following Figs.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Staining

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Graph in (A) represents the means (± SEM) of percent lung fibrosis. Representative Masson’s Trichrome stained lungs of PBS plus acetate buffer (B), BLM plus acetate buffer (C) and BLM plus AMD070 (D) treated mice. Note the intense cyan staining in the middle and lower panels indicative of collagen deposition in the lung parenchyma of BLM-treated mice. Bars represent 200 μm for the middle and 20 μM for the lower panel.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Staining

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Kaplan Meier plot of animals in the PBS plus acetate buffer, BLM plus acetate buffer and BLM plus AMD070 groups.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques:

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Concentrations of AMD070 in the plasma (A) and in the liver (B; ▲) or lung (B; ■) of C57BL/6 mice at various times following IP administration. Values shown are mean (± SEM) of n = 4/time point except n = 3 for the 2 hour time point for the liver calculation.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Clinical Proteomics

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Phoenix WinNonlin Noncompartmental analysis of AMD070 concentration in the plasma, lung and liver of C57BL/6 mice following IP or SC administration of AMD070 at 400 μg/mouse. Values for Cmax are provided as μg/mL for plasma and as μg/g for liver and lung. Values for AUC are provided as μg/mL for plasma and as μg/g for lung and liver.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Concentration Assay, Clinical Proteomics

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Cell counts in the blood of C57BL/6 mice at various times following the IP administration of AMD070 at 400 μg/mouse. A comparison of the numbers of WBCs(●; x 10 3 /μL), RBCs (■; x 10 6 /μL) and platelets (▲; x 10 6 /μL) are shown in panel (A) and are the means (± SEM) of 4 animals. Differential cell counts at various times are shown in panel (B) for lymphocytes (■), neutrophils (●), monocytes (▲) and eosinophils (▼) and are the means (± SEM) of 4 animals.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Comparison

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: Liver fibrosis was measured using Picosirius red and scored percent areas are shown in panel (A). Panels from each of the treatment groups contain representative Picrosirius Red stained left top lobe of livers from the oil plus PBS (B), CCl 4 plus PBS (C) and CCl 4 plus AMD070 (D) groups respectively. Bars represent 1 mm for the top image and 50 μm for the lower images in each panel.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: Staining

Journal: PLoS ONE

Article Title: Impact of a CXCL12/CXCR4 Antagonist in Bleomycin (BLM) Induced Pulmonary Fibrosis and Carbon Tetrachloride (CCl4) Induced Hepatic Fibrosis in Mice

doi: 10.1371/journal.pone.0151765

Figure Lengend Snippet: The relative transcription levels of Acta2 and Col1a1 are shown relative to housekeeping genes Gapdh (A) and Tbp (B). Serum AST levels are shown in panel (C). Prebleed is serum collected prior to CCl 4 and AMD070 treatment. Treated is serum collected one day after the last AMD070 or PBS treatment.

Article Snippet: The next day, AMD070 (Shanghai Haoyuan Chemexpress Co., 400 μg/mouse in 200 μL 30 mM acetate buffer) or 200 μL of 30 mM acetate buffer (pH 5) was administrated via oral gavage (PO) with a 20G feeding needle.

Techniques: